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Similar limitations had been discovered when five of seven flavourings were discovered to trigger cytotoxicity in human bronchial epithelial cells [87]. Results from in vitro research are normally agreement with the restricted variety of in vivo studies. For instance, in an analysis using main human umbilical vein endothelial cells (HUVEC) exposed to 11 commercially-available vapours, 5 were found to be acutely cytotoxic, and only three of those contained nicotine [24].